fitc plus anti mouse cd44 im7  (Proteintech)

Journal: Biomaterials Research

Article Title: External-Force-Offset Effects of ECM Coating Layers on hMSCs Subjected to External Physical Force

doi: 10.34133/bmr.0265

Figure Lengend Snippet: ECM coating using the layer-by-layer (LbL) technique was deposited on the cell surface. (A) Gelatin-RBITC/HyA-FITC-coated hMSCs via FACS analysis were shifted to the positive region (Gelatin-RBITC in red fluorescence and HyA–FITC in green fluorescence). (B) Mean fluorescence intensity (MFI) of the ECM coating layer on hMSC was changed with deposition of increasing numbers of layers based on FACS analysis ( n = 4).

Article Snippet: 160056, Mouse anti Human CD44: FITC Bio-Rad Formerly AbD Serotec), the ecto-5′-nucleotidase CD73(Lot.

Techniques: Fluorescence

Journal: Biomaterials Research

Article Title: External-Force-Offset Effects of ECM Coating Layers on hMSCs Subjected to External Physical Force

doi: 10.34133/bmr.0265

Figure Lengend Snippet: Surface morphological changes in bare hMSCs and ECM-hMSCs. (A) Confocal laser scanning microscopy (CLSM) images of Gelatin-RBITC (red)/HyA-FITC (green)-coated hMSCs. The nuclei were stained with 4′,6-diamidino-2-phenylindole (DAPI) in blue (magnification: ×400, scale bar: 20 μm). (B) The cell diameter of bare hMSCs and ECM-hMSCs was calculated using ImageJ based on the images of CLSM ( n = 50). (C) Scanning electron microscope (SEM) image depicting the cell surface morphology between the bare hMSCs and ECM-hMSCs independent experiments (inner image—magnification: ×5.0 k, scale bar: 10 μm; outer image—magnification: ×10.0 k, scale bar: 5 μm).

Article Snippet: 160056, Mouse anti Human CD44: FITC Bio-Rad Formerly AbD Serotec), the ecto-5′-nucleotidase CD73(Lot.

Techniques: Confocal Laser Scanning Microscopy, Staining, Microscopy

Journal: Stem Cell Research & Therapy

Article Title: Donor age and breed determine mesenchymal stromal cell characteristics

doi: 10.1186/s13287-025-04236-2

Figure Lengend Snippet: Immunophenotypic profile of bovine AT-MSCs. ( A ) Overview of MSC marker expression levels with bovine MSCs from all ages and both breeds expressing high CD29 and CD44 levels, variable CD34 and CD90 levels, and low CD14, CD45, CD79α, MHCII and CD73 levels. Quantitative data for markers ( B ) CD34, ( C ) CD44, and ( D ) CD90 are presented as the median percentage of positive cells, while the bars show the interquartile range. HF: Holstein Friesian; BB: Belgian Blue

Article Snippet: After blocking and washing, cells were incubated with a secondary phycoerythrin-cyanine 5 antibody (PE-Cy5, ThermoFisher, 1:100) together with the directly labeled primary monoclonal antibodies CD14-(R)PE (BioRad, CC-G33, 1:100) and CD44-FITC (BioRad, IL-A118, 1:10).

Techniques: Marker, Expressing